Guelph riding was created in 1976 from parts of Halton-Wentworth, Wellington, and Wellington-Grey ridings. It consisted initially of the Townships of Eramosa, Guelph, Pilkington and Puslinch and the City of Guelph in the County of Wellington.

The electoral district was abolished in 1987 when it was merged into Guelph-Wellington riding.

In 2003, Guelph riding was created again from parts of Guelph—Wellington. The riding now consists of the City of Guelph.